2D label-free imaging of resonant grating biochips in ultraviolet
نویسندگان
چکیده
منابع مشابه
2D label-free imaging of resonant grating biochips in ultraviolet.
2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm2 for proteins in air, and hence 10 pg/mm2 in water can be safely expected. A 320x256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biologica...
متن کاملLabel-free detection of protein-protein interactions on biochips.
The recent introduction of protein microarrays is now providing tools for the global profiling of biochemical activities. Snyder and co-workers, for example, used yeast protein chips to identify kinase substrates and lipid-binding proteins. Labaer and co-workers reported self-assembling protein microarrays to map pairwise interactions among 29 human DNA replication proteins. Any application of ...
متن کاملInterferometric silicon biochips for label and label-free DNA and protein microarrays.
Protein and DNA microarrays hold the promise to revolutionize the field of molecular diagnostics. Traditional microarray applications employ labeled detection strategies based on the use of fluorescent and chemiluminescent secondary antibodies. However, the development of high throughput, sensitive, label-free detection techniques is attracting attention as they do not require labeled reactants...
متن کاملDeep ultraviolet resonant Raman imaging of a cell.
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of R...
متن کاملDevelopment towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis
A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics Express
سال: 2010
ISSN: 1094-4087
DOI: 10.1364/oe.18.011472